ABSTRACT
The frequency of extremely early preterm birth (ERPR), their obstetric and perinatal outcomes, morphological features of the placenta in pregnant women in the city of Chelyabinsk and the Chelyabinsk region (CO) with a new coronavirus infection (NCI) in periods 1-2 and 3-4 waves of the COVID pandemic were studied. -19. The results obtained indicate that there is no increase in the frequency of ERPR in pregnant women with NCI. Delivery by caesarean section is associated mainly with the increase in acute respiratory distress syndrome (ARDS) of the mother. In the period of waves 3-4 of the COVID-19 pandemic, an increase in the perinatal mortality rate was noted compared to the period of waves 1-2 without statistical significance of these indicators. At the same time, antenatal fetal death during the 3rd-4th wave of the NCI pandemic was observed statistically significantly more often (p=0.033). The features of placental damage to the placentas in patients with NCI with ERPR and antenatal fetal death during the 3rd-4th wave of the pandemic are severe maternal and fetal vascular malperfusion and severe acute inflammatory lesions of the placenta (2,3 stages and 2,3 degrees).
ABSTRACT
The review presents data on the possible ways in which the SARS-CoV-2 and COVID-19 virus affects the female reproductive system and the already recorded negative consequences. Recommendations on pregnancy planning and the specifics of using hormonal contraceptive methods, as well as approaches to specific prevention of a new coronavirus infection from the standpoint of safety and preserving the reproductive health of women during the COVID-19 pandemic are outlined.
ABSTRACT
Under conditions of COVID-19 pandemic, considerable amounts of SARS-CoV-2 contained in household, municipal, and medical wastewaters inevitably reach natural water bodies. Possible preservation of virus infectivity in liquid environment is of a paramount epidemiological importance. Experiments demonstrated that SARS-CoV-2 is resistant to multiple freezing/thawing cycles and retains its infectivity in tap and river water for up to 2 days at 20°C and 7 days at 4°C. In natural milk, its viability is preserved in a refrigerator for 6 days. The exposure of aquarium fish to the virus-containing water fails to cause any infection.
Subject(s)
COVID-19 , Animals , Humans , Pandemics , SARS-CoV-2 , Wastewater , WaterABSTRACT
Background: The new coronavirus infection (NCI) COVID-19 in pregnant women occurs in various types from asymptomatic to extremely severe ones with the development of multiple organ failure and sepsis. SARS-CoV-2 infection occurs at the cellular level through viral binding to angiotensin-converting enzyme 2 (ACE2) receptors, the presence of which in the myocardium and vascular endothelial cells explains the mechanism of severe cardiovascular complications of NCI. The development of the latter has been demonstrated in few studies, whereas favorable outcomes for the mother and fetus in the presence of overall lung damage and acute coronary syndrome have not been reflected in the literature. Case report: The article describes and analyzes the favorable outcome of extremely severe NCI Covid-19 in a pregnant woman with overall lung damage, the development of acute myocarditis and myocardial infarction in the postpartum period. Conclusion: The timely referral of a pregnant woman with extremely severe NCI COVID-19 to the obstetric hospital of an infectious diseases hospital for patients with NCI COVID-19 for specialized medical care using a multidisciplinary approach, as well as the consideration of possible cardiac complications in this disease, their timely diagnosis and correction made it possible to effectively prevent an unfavorable outcome for the mother and fetus. © 2021, Bionika Media Ltd.. All rights reserved.
ABSTRACT
Different methods for producing bulk quantities of concentrated and purified SARS-CoV-2 for the use as antigens and for the research into COVID-19 biology were tested.
Subject(s)
COVID-19 Testing/methods , COVID-19/diagnosis , SARS-CoV-2/immunology , Viral Load/immunology , Virion/immunology , Animals , Antibodies, Neutralizing/isolation & purification , Antibodies, Neutralizing/metabolism , Antibodies, Viral/isolation & purification , Antibodies, Viral/metabolism , COVID-19/virology , Cell Line , Chlorocebus aethiops , Convalescence , Epithelial Cells/virology , Humans , Immune Sera/chemistry , SARS-CoV-2/genetics , SARS-CoV-2/growth & development , Swine , Vero Cells , Viral Load/genetics , Viral Proteins/immunology , Viral Proteins/isolation & purification , Viral Proteins/metabolism , Virion/genetics , Virion/growth & development , Virus ReplicationABSTRACT
We investigated the incidence of 15 respiratory viruses among 2991 children with acute respiratory infections in Novosibirsk city, Russia, prior to the COVID-19 pandemic (2013-2020). Viral infections were detected in 72.5% cases. The incidence of human coronavirus was 2% (Alphacoronaviruses, 63%; Betacoronaviruses, 37%).
Subject(s)
COVID-19 , Respiratory Tract Infections , COVID-19/epidemiology , Child , Child, Hospitalized , Hospitalization , Humans , Incidence , Pandemics , Respiratory Tract Infections/epidemiology , SARS-CoV-2ABSTRACT
Objective: isolation of coronavirus SARS-CoV-2 from clinical sample of patient with COVID-19 in Novosibirsk;obtaining a purified and inactivated viral antigen and study of its antigenic properties. Materials and methods: virus isolation was carried out in Vero cell culture from nasopharyngeal swab positive on SARS-CoV-2 RNA. The efficiency of SARS-CoV-2 replication in cell culture was assessed on the appearance of cytopathic effect (CPE) and the presence of viral RNA in cultural medium with reverse transcription – polymerase chain reaction (RT-PCR). Purification, concentration and inactivation of the viral preparation were carried out according to standard methods. The purity of the purified preparation and the profile of viral proteins were determined by electrophoresis in 10% polyacrylamide gel (PAG) with the addition of sodium dodecyl sulfate (SDS). The presence and specificity of viral proteins were detected using COVID-19 convalescent’s sera with enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Results: SARS-CoV-2/human/ RUS/Nsk-FRCFTM-1/2020 isolate was obtained after passage on Vero cells from a virus-containing clinical sample. A purified, concentrated, inactivated, whole-virion antigen was obtained. It contains three structural proteins: glycoprotein S (approximately 200 kDa), nucleoprotein N (48 kDa), and matrix protein M (20-25 kDa). All viral proteins were detected with serum antibodies of COVID-19 convalescents. Conclusion: SARS-CoV-2 coronavirus can be isolated in Vero cell culture. The antigenic specificity of the three structural viral proteins (S, N, and M) is preserved in the purified inactivated viral preparation. The inactivated whole-virion antigen of SARS-CoV-2/human/RUS/Nsk-FRCFTM-1/2020 isolate can be used to study the antigenic immunomodulating properties of viral proteins, to obtain immune sera of laboratory animals, and also as a component of test systems for the detection of specific antibodies with ELISA and immunoblotting.